Not every assay needs the same substrate. But every assay needs the right one.
Kementec’s TMB Crew is a complete portfolio of chromogenic HRP substrates developed to support different ELISA and immunoassay needs - from everyday reliability to high sensitivity, automation, challenging conditions, and safer substrate replacement.
Why choose Kementec’s TMB products?
· Very low background for clean and reliable signals
· Strong color development for clear HRP detection
· Excellent stop stability for consistent OD readings
· High batch-to-batch reproducibility
· Reliable performance across different ELISA and immunoassay workflows
· Aqueous formulations without organic solvents
· Options for standard assays, high-sensitivity assays, automated systems, and safer substrate replacement
· Prestained versions available for visual control during pipetting and setup
· Technical support to help match the right TMB substrate to the right assay setup
· Free samples available if you want to evaluate performance in your own workflow
Why Kementec is the right partner
Kementec Solutions is a Denmark-based manufacturer and supplier with more than 35 years of experience supporting researchers, assay developers, diagnostic companies, and laboratories worldwide. Known for high-quality immunoassay solutions, consistent supply, and strong technical expertise, Kementec values long-term, mutual business relationships and aims to be more than a supplier - they aim to be a trusted partner.
For companies looking for dependable TMB substrates, Kementec offers a strong combination of performance, stability, flexibility, and support. Their products are developed to help customers achieve reliable assay results, efficient workflows, and confidence from development to routine manufacturing or testing.
Why the right TMB substrate matters
The right TMB substrate can help laboratories achieve better control from development to routine use. It can help reduce troubleshooting, support cleaner signal interpretation, improve reproducibility, and make workflows more robust. For laboratories working with ELISA, HRP-based detection, assay optimization, diagnostic development, or manufacturing, this level of control can support more reliable decision-making and more efficient assay performance.
Kementec’s TMB Crew is designed to give laboratories options - not just one substrate, but a full portfolio with different performance profiles.
TMB ONE: The gold standard
TMB ONE is the original, trusted choice for laboratories that need proven stability and dependable performance. With more than 35 years of proven use, TMB ONE has become a recognized standard for chromogenic HRP detection.
Benefits of TMB ONE:
• Proven and trusted performance
• Extremely stable and reliable
• Very low lot-to-lot variation
• Strong choice when consistency matters most
• Ideal for laboratories that want predictable, dependable results
When an assay needs a stable and predictable substrate that performs without unnecessary complexity, TMB ONE is a strong starting point. It is the dependable member of the crew - the one to choose when reliability is the priority.
TMB PLUS2®: The all-rounder
TMB PLUS2® is designed for laboratories that need a versatile substrate with higher activity than TMB ONE, while still maintaining very low background and robust performance.
Benefits of TMB PLUS2®:
• Higher activity than TMB ONE
• Very low background
• Fast and robust performance
• Suitable for many ELISA and immunoassay setups
• A strong default choice when both speed and reliability are needed
For many assay setups, TMB PLUS2® can serve as an excellent all-rounder. It offers a useful balance of speed, sensitivity, and reliability, making it suitable for a wide range of applications and everyday assay workflows.
TMB X-tra: The speed demon
Some assays do not have time to wait. TMB X-tra is designed for fast reaction kinetics, high sensitivity, and strong signal development - even with short incubation times.
Benefits of TMB X-tra:
• Very high sensitivity
• Strong signal development
• Fast reaction kinetics
• Works well with short incubation times
• Easy to use in workflows where speed matters
For laboratories optimizing incubation time, improving workflow efficiency, or working with assays that require high sensitivity, TMB X-tra offers a powerful option. Less waiting. More signal.
TMB X-tnd®: The indestructible
Some assay environments are more demanding than others. TMB X-tnd® is built for robustness, automation, and extreme assay conditions.
Benefits of TMB X-tnd®:
• No precipitation, even at very high OD levels
• Resistant to wash buffer leftovers
• Strong choice for automated systems
• High robustness under demanding conditions
• Suitable for high-throughput workflows and challenging assay setups
For laboratories working with automation, challenging protocols, or high signal levels, TMB X-tnd® provides an added layer of reliability. When an assay needs to withstand tough conditions, this is the member of the crew built for the job.
TMB EASE: The safe switch
For laboratories using OPD, TMB EASE offers a safer and practical alternative. It is designed as a non-carcinogenic 1:1 replacement for OPD and supports a smooth transition from existing protocols.
Benefits of TMB EASE:
• Non-carcinogenic alternative to OPD
• 1:1 replacement for existing OPD workflows
• Supports a smoother transition to safer chemistry
• Stable and easy to use
• Helps improve laboratory safety without redesigning the workflow
For teams that want to move away from more hazardous substrate chemistry while maintaining a familiar assay process, TMB EASE is a simple and effective switch. Same workflow. Better choice.
Prestained TMB: Visual control when it matters
In assay workflows, visual confirmation can reduce uncertainty during setup.
Kementec offers prestained versions of TMB ONE, TMB PLUS2®, and TMB X-tra. These pink versions provide clear visual control during pipetting and help users confirm that the substrate has been added correctly.
The indicator disappears after stop solution is added, so it does not interfere with the final readout. Prestained TMB substrates support traceable pipetting, clearer workflow control, and greater confidence.
Find the right TMB for your assay
Kementec’s TMB Crew is designed to help laboratories match the right substrate to the right assay. Choosing the right substrate can improve assay performance, reduce troubleshooting, and support a more reliable workflow from development to routine use.
To help laboratories evaluate the best fit, Kementec offers free samples of its TMB products. Reach out to Kementec to request a free sample and discuss which TMB substrate is best suited for your assay setup: https://kementec.com/contact-us/
Your assay deserves more than just a substrate.
It deserves the right crew.
