In Vitro Diagnostic Industry in China - Blood Typing Instruments and Reagents XIV

4.2.2 Development Trend of Blood Typing Reagent Technologies

It can be seen from analysis on major Blood typing reagents-related patent applications published in the 3 years between 2018 and 2021 that directions of technical researches mainly focused on rare specific antigen test, PCR genotyping test technique and improvement of existing test methods. It is foreseeable that development trend of Blood typing reagent technologies is mainly reflected by improvement of Blood typing accuracy, increase in Blood typing efficiency and reduction of test time; improvement of Blood typing sensitivity and prevention of missed test; evaluation of antibody titer determination and other aspects.

In terms of improvement of Blood typing accuracy, different techniques mainly focus on provision of genotyping-based ABO blood typing, and acquirement of target segments through design of specific primers and use of single-tube multiplex PCR amplified reaction, which allow doctors to acquire more precise and comprehensive blood group results; in respect of reduction of test time and improvement of test efficiency, multiplex PCR method is introduced to ABO blood group genotyping, which can reduce number of PCR reactions of a single person-time in ABO antigen genotyping, reduce human and reagent cost of test, and increase test throughput; as for evaluation of antibody titer test capability, blood group antibody titer test capability of different laboratories engaged in test are judged, and accuracy of blood group antibody test capability verification results are improved based on media and methods used by them; in case of improvement of sensitivity and prevention of missed test, technologies are mainly distributed in the fields of use of antigen and antibody immunoreaction for high strength adsorption and bonding of erythrocytes as well as use of freeze-drying technique and two-step indication system, which can solve existing clinical difficulties.

Last: In Vitro Diagnostic Industry in China - Blood Typing Instruments and Reagents XIII